Step 1: Online Tutorials: It is essential for users to watch these tutorial videos prior to face-to-face training. Cancellations with less than 24 hours’ notice will be charged 50% of the booked time, and no-shows will be charged in full. Users can cancel their bookings by contacting us up to 24 hours before their sort. Users will be charged based on the time booked or time used, whichever is longer.
Minimum booking is 1 hour, after which users will be charged in 30-minute increments. **Please be aware that your booking time includes 20 min for machine set up, and 10 min after-sort cleaning, and an additional 5 - 10 mins if you require purity checks after sorting. Please indicate if you are sorting primary human tissue or other PC2 classified cells.Īll cells for sorting must be sieved through 40 µm mesh prior to sorting. However, the larger the nozzle size, the slower the sorter can sort, and therefore, the fewer cells can be sorted during your booking.īooking can only be made by contacting us. Selecting the correct nozzle size will ensure sorting efficiency, reduce cell death and prevent blockages. Larger cells, such as HeLa (~20 μm) need a 100 μm nozzle. If you want to sort lymphocytes (around 10 μm) then a 70 μm nozzle is used (this is the smallest size). The rule of thumb when sorting is to use a nozzle that is 4 times larger than your cell size. Three sizes of nozzles are available: 70, 85, and 100 μm. It is important to plan a meeting to discuss your sorting needs before experiments. Bookings are essential.Īll cells must be sieved through 40 µm mesh prior to analysis.Ĭharging starts from the time booked, so it is in your interest to arrive on time.Ĭancellations can be made via the online booking system with between 24-48 hours’ notice.Ĭancellations with less than 24 hours’ notice will be charged 50% of the booked time, minimum of 1 hour, and no-shows will be charged in full.Īvailability: The Sorter will be operated by the core. For training and assisted usage please book.īookings can be made via online Outlook calendar using these Instructions.The calendar names are *G HEALTH Medicine Lab Eqpmt ka4.308 SOMFlow and *G HEALTH Medicine Lab Eqpmt ka4.327 FlowJo Dongle Minimum booking is 1 hour, followed by 30 minute increments thereafter. For more information please do not hesitate to contact us.īooking Conditions & Policy – SOM Flow Cytometry Facility (SOMFlow)įor all enquiries regarding the Flow Cytometry equipment, please contact us.Īvailability: The analyser is available for researchers to operate 24/7 following training and reaching a high level of competence. Please complete the Use and Training Request form along with reading the SOP's. Cell sorter is operated by the core that provides services catering for both animal and human cell sorting in a PC2 environment.Īll users must be familiar with the booking rules, associated usage costs, usage guidelines and standard operating procedures (SOPs). Training is provided for analyser, and analyser is operated by qualified and licensed users. Our PC2 facility currently houses one BD FACSCanto TM II analyser (3-Laser, 8-Color) and one BD FACSAria TM III sorter (4-Laser, 12-Color). You can check to see that all your samples have been included by opening the file, and plotting some parameter against 'SampleID' (or your custom parameter).SOMFlow offers comprehensive training and education, experimental design and protocol guidance specifically targeting effective data generation and interpretation in the field of flow cytometry. Alternatively, you can add additional keywords, and add them as additional parameters to the concatenated file.Ĭhoose an output location – best to create a folder within your existing experiment folder.Ĭlick 'Concatenate' on the bottom right, and wait for the new FCS file to be created.ĭrag the new FCS file into that workspace, and save the workspace in that folder. By default, each sample will be separated in a new parameter called 'SampleID', where samples are organised alphabetically ( I think it's alphabetical). We will be concatenating FCS files, so we can just use 'all uncompensated parameters'. In the new window, select 'Concatenate' at the top. Right click on one of the nodes, and select 'Export / Concatenate Populations'. Select your POPI and use the 'select equivalent nodes' tool under the 'edit' menu. Gate to your population of interest (POI) in the first sample.Īdd the gates to the group, and adjust for all samples, as per a normal analysis in FlowJo. To start with, we want to pull our FCS files into FlowJo.
0 kommentar(er)
